Christina B. Wölwer
Luke B. Pase
Imogen A. Elsum
Krystle Y. B. Lim
Carl Walkley, Australian Catholic UniversityFollow
Sarah M. Russell
Patrick O. Humbert
Wölwer, C. B, Gödde, N., Pase, L. B, Elsum, I. A, Lim, K. Y, Sacirbegovic, F., Walkley, C., Ellis, S., Ohno, S., Matsuzaki, F., Russell, S. M & Humbert, PO. (2017). The asymmetric cell division regulators par3, scribble and PINS/GPSM2 are not essential for erythroid development or enucleation. PLoS One,12(1), H. Wang. 1-14. United States of America: Public Library of Science (PLoS). Retrieved from https://doi.org/10.1371/journal.pone.0170295
Erythroid enucleation is the process by which the future red blood cell disposes of its nucleus prior to entering the blood stream. This key event during red blood cell development has been likened to an asymmetric cell division (ACD), by which the enucleating erythroblast divides into two very different daughter cells of alternate molecular composition, a nucleated cell that will be removed by associated macrophages, and the reticulocyte that will mature to the definitive erythrocyte. Here we investigated gene expression of members of the Par, Scribble and Pins/Gpsm2 asymmetric cell division complexes in erythroid cells, and functionally tested their role in erythroid enucleation in vivo and ex vivo. Despite their roles in regulating ACD in other contexts, we found that these polarity regulators are not essential for erythroid enucleation, nor for erythroid development in vivo. Together our results put into question a role for cell polarity and asymmetric cell division in erythroid enucleation.
Mary MacKillop Institute for Health Research
Open Access Journal Article
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