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Excess accumulation of lipids in nonadipose tissues such asskeletal muscle and liver has been implicated in the develop-ment of obesity-related disorders, but the cause of this ec-topic lipid overload remains unknown. The aim of this studywas to determine in vivo postprandial lipid partitioning in ratskeletal muscle and liver, using localized1H-[13C] magneticresonance spectroscopy in combination with the oral admin-istration of13C-labeled lipids. Six rats were measured atbaseline and 5 and 24 h after administration of 400 mg[U-13C]-labeled algal lipids. Five hours after administration,fractional13C enrichments of the lipid pools in muscle andliver were increased 3.9-fold and 4.6-fold (P < 0.05), respec-tively, indicating that part of the ingested lipids had beentaken up by muscle and liver tissue. At 24 h, fractional13Cenrichments of muscle and liver lipids were decreased 1.6-fold and 2.2-fold (P < 0.05), respectively, compared with the 5h values. This can be interpreted as a depletion of13C-labeledlipids from the intracellular lipid pools as a consequence oflipid turnover. In conclusion, the novel application of1H-[13C]magnetic resonance spectroscopy in combination with theoral administration of13C-labeled lipids is applicable for thelongitudinal assessment of in vivo lipid partitioning betweenmultiple tissues. Magn Reson Med 68:997–1006, 2012.VC2011Wiley Periodicals, Inc.Key words: magnetic resonance spectroscopy; muscle lipids;liver lipids; carbon-13


Mary MacKillop Institute for Health Research

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