Impact of protein coingestion on muscle protein synthesis during continuous endurance type exercise

Journal article


Beelen, M., Zorenc, Antoine H., Pennings, Bart, Senden, Joan M., Kuipers, Harm and Van Loon, Luc J.C.. (2011). Impact of protein coingestion on muscle protein synthesis during continuous endurance type exercise. American Journal of Physiology - Endocrinology and Metabolism. 300(6), pp. 945 - 954. https://doi.org/10.1152/ajpendo.00446.2010
AuthorsBeelen, M., Zorenc, Antoine H., Pennings, Bart, Senden, Joan M., Kuipers, Harm and Van Loon, Luc J.C.
Abstract

This study investigates the impact of protein coingestion with carbohydrate on muscle protein synthesis during endurance type exercise. Twelve healthy male cyclists were studied during 2 h of fasted rest followed by 2 h of continuous cycling at 55% Wmax. During exercise, subjects received either 1.0 g·kg−1·h−1 carbohydrate ( CHO ) or 0.8 g·kg−1·h−1 carbohydrate with 0.2 g·kg−1·h−1 protein hydrolysate ( CHO+PRO ). Continuous intravenous infusions with l-[ring-13C6]phenylalanine and l-[ring-2H2]tyrosine were applied, and blood and muscle biopsies were collected to assess whole body protein turnover and muscle protein synthesis rates at rest and during exercise conditions. Protein coingestion stimulated whole body protein synthesis and oxidation rates during exercise by 22 ± 3 and 70 ± 17%, respectively ( P < 0.01 ). Whole body protein breakdown rates did not differ between experiments. As a consequence, whole body net protein balance was slightly negative in CHO and positive in the CHO+PRO treatment ( −4.9 ± 0.3 vs. 8.0 ± 0.3 μmol Phe·kg−1·h−1, respectively, P < 0.01 ). Mixed muscle protein fractional synthetic rates ( FSR ) were higher during exercise compared with resting conditions ( 0.058 ± 0.006 vs. 0.035 ± 0.006%/h in CHO and 0.070 ± 0.011 vs. 0.038 ± 0.005%/h in the CHO+PRO treatment, respectively, P < 0.05 ). FSR during exercise did not differ between experiments ( P = 0.46 ). We conclude that muscle protein synthesis is stimulated during continuous endurance type exercise activities when carbohydrate with or without protein is ingested. Protein coingestion does not further increase muscle protein synthesis rates during continuous endurance type exercise.

Year2011
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Journal citation300 (6), pp. 945 - 954
PublisherAmerican Physiological Society
ISSN0193-1849
Digital Object Identifier (DOI)https://doi.org/10.1152/ajpendo.00446.2010
Scopus EID2-s2.0-79957662834
Page range945 - 954
Research GroupMary MacKillop Institute for Health Research
Publisher's version
File Access Level
Controlled
Place of publicationUnited States
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