Increasing insulin availability does not augment postprandial muscle protein synthesis rates in healthy young and older men

Journal article


Groen, Bart B. L., Horstman, Astrid M. H., Hamer, Henrike M., de Haan, Michiel, van Kranenburg, Janneau, Bierau, Jörgen, Poeze, Martijn, Wodzig, Will K. W. H., Rasmussen, Blake B. and van Loon, Luc J. C.. (2016). Increasing insulin availability does not augment postprandial muscle protein synthesis rates in healthy young and older men. The Journal of Clinical Endocrinology and Metabolism. 101(11), pp. 3978 - 3988. https://doi.org/10.1210/jc.2016-1436
AuthorsGroen, Bart B. L., Horstman, Astrid M. H., Hamer, Henrike M., de Haan, Michiel, van Kranenburg, Janneau, Bierau, Jörgen, Poeze, Martijn, Wodzig, Will K. W. H., Rasmussen, Blake B. and van Loon, Luc J. C.
Abstract

Context: Skeletal muscle protein synthesis is highly responsive to food intake. It has been suggested that the postprandial increase in circulating insulin modulates the muscle protein synthetic response to feeding. Objective: The objective of the study was to investigate whether a greater postprandial rise in circulating insulin level increases amino acid uptake in muscle and augments postprandial muscle protein synthesis rates. Participants and Design: Forty-eight healthy young ( age 22 ± 1 y; body mass index 22.0 ± 0.3 kg/m2 ) and older males ( age 68 ± 1 y; body mass index 26.3 ± 0.4 kg/m2 ) ingested 20 g intrinsically L-[1-13C]-leucine- and L-[1-13C]-phenylalanine-labeled casein protein with or without local insulin infusion. Primed continuous infusions of L-[1-13C]-leucine and L-[ring-2H5]-phenylalanine were applied, with arterial and venous blood samples and muscle biopsies being collected during a 5-hour postprandial period. Results: Insulin administration did not increase overall leg blood flow ( P = .509 ) but increased amino acid uptake over the leg in both young and older subjects ( P = .003 ). The greater amino acid uptake over the leg did not further increase postprandial muscle protein synthesis rates ( 0.050% ± 0.006% and 0.037% ± 0.004% per hour vs 0.044% ± 0.004% and 0.037% ± 0.002% per hour in the insulin-stimulated vs control condition in the young and older groups, respectively; P = .804 ) and did not affect postprandial deposition of dietary protein-derived amino acids in de novo muscle protein ( P = .872 ). Conclusion: Greater postprandial plasma insulin availability stimulates amino acid uptake over the leg but does not further augment postprandial muscle protein synthesis rates or stimulate the postprandial deposition of protein derived amino acids into de novo muscle protein in healthy young and older men.

Year2016
JournalThe Journal of Clinical Endocrinology and Metabolism
Journal citation101 (11), pp. 3978 - 3988
PublisherEndocrine Society
ISSN0021-972X
Digital Object Identifier (DOI)https://doi.org/10.1210/jc.2016-1436
Scopus EID2-s2.0-84994860262
Page range3978 - 3988
Research GroupMary MacKillop Institute for Health Research
Publisher's version
File Access Level
Controlled
Place of publicationUnited States
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