A protocol for measurement of noncoding RNA in human serum

Journal article

Taylor, Caroline J., Satoor, Sarang N., Ranjan, Amaresh K., Pereira e Cotta, Maria V. and Joglekar, Mugdha V.. (2012). A protocol for measurement of noncoding RNA in human serum. Experimental Diabetes Research. 2012, pp. 1 - 4. https://doi.org/10.1155/2012/168368
AuthorsTaylor, Caroline J., Satoor, Sarang N., Ranjan, Amaresh K., Pereira e Cotta, Maria V. and Joglekar, Mugdha V.
Abstract

MicroRNAs (miRNAs) are small noncoding RNAs that act as regulators of gene expression by targeting mature messenger RNAs. Following the initial report of the presence of miRNAs in serum and plasma a number of studies have successfully demonstrated the use of these miRNAs as biomarkers of disease. Currently, there are many methods of isolating total RNA from liquid samples. Here, we describe a simple, cost effective method for extraction of RNA from human serum as well as subsequent real time PCR analysis of miRNA levels.

Year2012
JournalExperimental Diabetes Research
Journal citation2012, pp. 1 - 4
PublisherHindawi Publishing Corporation
ISSN1687-5214
Digital Object Identifier (DOI)https://doi.org/10.1155/2012/168368
Scopus EID2-s2.0-84864928987
Open accessOpen access
Page range1 - 4
Research GroupSports Performance, Recovery, Injury and New Technologies (SPRINT) Research Centre
Publisher's version
License
Place of publicationUnited States of America
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